An Indirect ELISA for Urinary Gonadotropins Using Immobilized Human Menopausal Gonadotropin

Development of an indirect ELISA for bovine mastitis using Sip protein of Streptococcus agalactiae

The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) and expressed as a recombinant protein in E. coli BL21 (DE3). An indirect enzyme linked immunosorbent assay (ELISA) was developed using the purified Sip protein as a coating antigen, w...

Fusion proteins of human gonadotropins and gonadotropin-receptor complexes

Three glycoprotein hormones comprise the family of gonadotropins in humans, luteinizing hormone (LH), chorionic gonadotropin (CG), and fol l icle-stimulating hormone (FSH), composed of a common a subunit and a hormone-specific � subunit. These heterodimers act via two G protein-coupled receptors, the LH receptor (LHR) and the FSH receptor (FSHR), that are expressed on gonadal cells. The � subun...

development of an indirect elisa for bovine mastitis using sip protein of streptococcus agalactiae

the sip gene encoding for a conserved highly immunogenic surface protein of streptococcus agalactiae was amplified using polymerase chain reaction (pcr) and subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in e. coli bl21 (de3). an indirect enzyme linked immunosorbent assay (elisa) was developed using the purified sip protein as a coating antigen, w...

Human Menopausal Gonadotropin versus Recombinant FSH in Polycystic Ovary Syndrome Patients Undergoing In Vitro Fertilization

Endocrine profile following stimulation with recombinant follicle stimulating hormone and luteinizing hormone versus highly purified human menopausal gonadotropin

BACKGROUND Luteinizing hormone (LH) activity in human menopausal gonadotropin (hMG) preparations is derived from human chorionic gonadotropin (hCG) rather than LH. Therefore, we aimed to determine whether there are similarities in the endocrine and follicular profiles of serum and follicular fluid from controlled ovarian stimulation with the recombinant gonadotropins follicle-stimulating hormon...